DETERMINATION OF TOTAL IMMUNOGLOBULIN CLASSES M AND G – INFORMATIVE METHOD OF DIAGNOSIS OF GIARDIASIS, ASCARIASIS
Aim. Conducting a study on the presence of total immunoglobulin classes M and G to antigens of ascarids, giardia in human blood by immunoenzyme analysis.
Materials and methods. The study involved 35 family members living in the city of Dushanbe GTSZ N»1 without complaints. There were 19 (54%) men and 16 (46.0%) – women.
Results. During the study of blood for presence of total immunoglobulin classes M and G to antigens of Ascaris, by ELISA positive results were found in 14 (31,4%) members of the family: 8 women (57,1%), 6 males (42,8%); 8 (57,1%) had organized (working) population, 6 (42,8%) of unorganized (non-working). In our study, the titer of antibody was determined to determine the duration of the disease. By titration of AT 1:100 – 5 (35,7%), AT-1: 200 – 9 (64,9%). In the study of antibodies to roundworm found that the infestation is more common among females, have organized a contingent of the population. Of those studied, 64.9% of family members infected with ascariasis.
Blood test for the presence of total immunoglobulin classes M and G lamblia antigens by ELISA revealed positive responses in 10 persons (28,5%) among the surveyed family members, of whom – 4 women (40,0 %), 6 men (60%). Of the total number of persons with a positive result to the antigens of Giardia organized (working) – 8 (80%), fugitive (non-working) – 2 people (20%). According to the definition of titer: AT-1:200 – 8 people (80%), AT-1:800 – 2 person (20%). Of the total study 20% of family members infected with Giardia, the prevalence of greater among organized among males.
Conclusion. Concurrent use of koprological and serological methods of diagnosis allows more reliably and efficiently identify ascariasis, giardiasis.